Kimura K, Ogawa K, Sato K, Tokuhara S, Otsuka M, Matsunaga Y, Tsubura E
Scand J Immunol. 1984 Jun;19(6):513-20. doi: 10.1111/j.1365-3083.1984.tb00962.x.
Natural killer (NK) cell activity in experimental murine amyloidosis was studied. In CBA/J mice, which show a high incidence of amyloidosis, NK activity was significantly decreased after 1 week of casein treatment. In C3H mice, which show a low incidence of amyloidosis, NK activity was not changed by casein treatment. Pretreatment with lipopolysaccharide in vivo enhanced the NK activities in CBA/J and C3H mice. These increases were not observed after casein treatment. The lowered NK activity of cells from CBA/J mice after casein treatment was restored to the normal range by indomethacine in vitro. Depletion of adherent cells from the spleen cells treated with casein had no effect on NK activity. Single-cell assay showed that casein treatment impaired the killing but not the binding of NK cells to target cells. After casein treatment, the splenic serum amyloid A (SAA) level gradually increased in CBA/J mice but remained low in C3H mice. NK activity was suppressed by the addition of serum obtained from CBA/J mice treated with casein but not by normal control serum. And partially purified AA protein obtained from the spleen of CBA/J mice treated with casein also suppressed NK activity in vitro.
对实验性小鼠淀粉样变性中的自然杀伤（NK）细胞活性进行了研究。在淀粉样变性发病率较高的CBA/J小鼠中，酪蛋白处理1周后NK活性显著降低。在淀粉样变性发病率较低的C3H小鼠中，酪蛋白处理未改变NK活性。体内用脂多糖预处理可增强CBA/J和C3H小鼠的NK活性。酪蛋白处理后未观察到这些增加。酪蛋白处理后CBA/J小鼠细胞降低的NK活性在体外被吲哚美辛恢复到正常范围。用酪蛋白处理的脾细胞中黏附细胞的耗竭对NK活性没有影响。单细胞分析表明，酪蛋白处理损害了NK细胞对靶细胞的杀伤作用，但不影响其结合。酪蛋白处理后，CBA/J小鼠脾脏血清淀粉样蛋白A（SAA）水平逐渐升高，而C3H小鼠中则保持较低水平。添加用酪蛋白处理的CBA/J小鼠的血清可抑制NK活性，而正常对照血清则无此作用。从用酪蛋白处理的CBA/J小鼠脾脏中获得的部分纯化的AA蛋白在体外也抑制NK活性。
