Kusanagi T
Teratology. 1984 Apr;29(2):281-6. doi: 10.1002/tera.1420290214.
C57BL/6 mice were treated subcutaneously with triamcinolone acetonide in a single dose of 2.5-15.0 mg/kg once on days 6-15 of pregnancy (vaginal plug = day 0) and the palate of their fetuses was examined at term. The sensitive stages of palatal slit and cleft palate induction were studied and dose-response relations on days 9 and 12 of pregnancy were analyzed by the log-probit transformation method and compared. The test of significant increase in the frequencies of palatal slit and cleft palate showed that the sensitive period of palatal slit began earlier and ended later than that of cleft palate. On day 9 of pregnancy, the slope of the palatal slit response was similar to that of the cleft palate response, but the median effective dose of the former was lower than that of the latter. The slope of the palatal slit response was similar on days 9 and 12; however, the median effective dose was significantly greater on day 9. By contrast, the slope of the cleft palate response on day 12 was significantly different from that on day 9. The results of these dose-response analyses suggest that the underlying mechanism may be the same in palatal slit induction on days 9 and 12 and in cleft palate induction on day 9; the mechanism underlying the cleft palate response on day 12 is different from that of the other three responses, and there is more than one mechanism for cleft palate induced by triamcinolone.
在妊娠第6 - 15天（阴道栓形成日 = 第0天），对C57BL / 6小鼠皮下注射单剂量2.5 - 15.0 mg / kg的曲安奈德一次，足月时检查其胎儿的腭部。研究了腭裂和唇腭裂诱导的敏感阶段，并采用对数概率转换法分析并比较了妊娠第9天和第12天的剂量 - 反应关系。腭裂和唇腭裂频率显著增加的测试表明，腭裂的敏感时期开始得比唇腭裂早且结束得晚。在妊娠第9天，腭裂反应的斜率与唇腭裂反应的斜率相似，但前者的半数有效剂量低于后者。腭裂反应在第9天和第12天的斜率相似；然而，第9天的半数有效剂量显著更大。相比之下，第12天唇腭裂反应的斜率与第9天显著不同。这些剂量 - 反应分析结果表明，第9天和第12天腭裂诱导以及第9天唇腭裂诱导的潜在机制可能相同；第12天唇腭裂反应的潜在机制与其他三种反应不同，并且曲安奈德诱导唇腭裂存在多种机制。
