Gandy J, Ali F A, Hasegawa L, Imamura T
Toxicology. 1984 Jul;32(1):37-46. doi: 10.1016/0300-483x(84)90032-5.
A single oral administration of O, O, S-trimethyl phosphorothioate (OOS-Me), an impurity in widely used organophosphorus insecticides, causes delayed toxicity (delayed death) which is accompanied by morphological changes in the bronchiolar epithelium of rat lungs. A series of simple O,O-dimethyl and O,O-diethyl S-alkyl phosphorothioate esters, which induce delayed toxicity, were examined for their effect on rat bronchiolar epithelium. The structural analogues synthesized and tested include O, O-dimethyl S-ethyl phosphorothioate, O,O-dimethyl S-isopropyl phosphorothioate, O,O,S-triethyl phosphorothioate, and O,O-diethyl S-methyl phosphorothioate. The present investigation demonstrated that these analogues of OOS-Me which cause delayed toxicity produce body weight loss, accompanied by morphological alterations of terminal bronchiolar epithelium, i.e. loss of the apical bulge of non-ciliated Clara cells. Another impurity which produces delayed toxicity, O,S,S-trimethyl phosphorodithioate, was also capable of producing similar effects at near the LD50 level.
广泛使用的有机磷杀虫剂中的一种杂质O,O,S-三甲基硫代磷酸酯（OOS-Me）单次口服会导致延迟性毒性（延迟死亡），并伴有大鼠肺细支气管上皮的形态学变化。对一系列会引发延迟性毒性的简单O,O-二甲基和O,O-二乙基S-烷基硫代磷酸酯对大鼠细支气管上皮的影响进行了研究。合成并测试的结构类似物包括O,O-二甲基S-乙基硫代磷酸酯、O,O-二甲基S-异丙基硫代磷酸酯、O,O,S-三乙基硫代磷酸酯和O,O-二乙基S-甲基硫代磷酸酯。本研究表明，这些导致延迟性毒性的OOS-Me类似物会导致体重减轻，并伴有终末细支气管上皮的形态学改变，即无纤毛克拉拉细胞顶端凸起的丧失。另一种产生延迟性毒性的杂质O,S,S-三甲基二硫代磷酸酯在接近半数致死剂量水平时也能够产生类似的效果。
