肌酸激酶同工酶的测定：咖啡因和视黄酸对胎鼠肢体中肌酸激酶水平的影响

Estimation of creatine kinase isoenzymes: the effects of caffeine and retinoic acid on CK levels in fetal mouse limbs.

作者信息

Kwasigroch T E, Skalko R G

出版信息

Toxicol Lett. 1984 Jun;21(3):339-47. doi: 10.1016/0378-4274(84)90094-8.

Abstract

Caffeine and retinoic acid were examined for effects upon limb morphogenesis and upon creatine kinase (CK) as a measure of limb myogenesis. Caffeine at 200 mg/kg, i.p., on E11 produced a low level of forelimb (1.2%) and hindlimb (2.0%) defects. Retinoic acid, at 50 mg/kg given orally as an oily suspension, induced a high level of reduction deformities. Hindlimbs (100%) were affected more than forelimbs (88%). Limbs (E16) were examined for CK isoenzymes using DEAE-Sephacel column chromatography. Untreated limbs had 88.04% skeletal muscle (MM), 6.98% hybrid (MB) and 5.08% brain (BB) CK isoenzyme. Caffeine had no effect. However, retinoic acid increased MM-CK to 92.67%, and decreased BB-CK to 2.24%. This is the first evidence that suggests that retinoic acid may modify the phenotypic expression of developing muscle.

摘要

研究了咖啡因和视黄酸对肢体形态发生的影响以及对肌酸激酶（CK）的影响，以此作为肢体肌生成的一个指标。在胚胎第11天腹腔注射200mg/kg的咖啡因，导致前肢（1.2%）和后肢（2.0%）出现低水平的畸形。以油悬液形式口服给予50mg/kg的视黄酸，会引发高水平的肢体发育不全畸形。后肢（100%）比前肢（88%）受影响更严重。使用DEAE - 琼脂糖凝胶柱色谱法对胚胎第16天的肢体进行CK同工酶检测。未处理的肢体中，骨骼肌（MM）型CK同工酶占88.04%，混合型（MB）占6.98%，脑型（BB）占5.08%。咖啡因没有影响。然而，视黄酸使MM - CK增加到92.67%，并使BB - CK减少到2.24%。这是首个表明视黄酸可能改变发育中肌肉表型表达的证据。