Jongejan F, Morzaria S P, Shariff O A, Abdalla H M
Vet Res Commun. 1984 May;8(2):141-5. doi: 10.1007/BF02214705.
Heartwater was diagnosed in sheep at the Umm Banein Livestock Research Station, Blue Nile Province, Sudan. Cowdria ruminantium was isolated from sheep by subinoculation of blood into a goat. Adult Amblyomma lepidum ticks fed as nymphs on this goat transmitted heartwater to a Friesian (Bos taurus) calf. Brain stabilates from this calf, either snap-frozen into liquid nitrogen or frozen slowly in a deepfreezer at -70 degrees C, with DMSO as cryoprotectant, were infective to cattle when inoculated subcutaneously.
在苏丹青尼罗省乌姆巴奈因畜牧研究站的绵羊中诊断出心水病。通过将血液接种到山羊体内，从绵羊中分离出反刍兽考德里氏体。以这只山羊为若虫宿主进食的成年微小扇头蜱将心水病传播给了一头弗里斯兰（Bos taurus）小牛。这头小牛的脑匀浆，要么速冻于液氮中，要么在-70摄氏度的冰柜中缓慢冷冻，并添加二甲基亚砜作为冷冻保护剂，皮下接种时对牛具有感染性。
