Ngumi P N, Rumberia R M, Williamson S M, Sumption K J, Lesan A C, Kariuki D P
National Veterinary Research Centre, Kikuyu, Kenya.
Vet Rec. 1997 Jan 4;140(1):13-6. doi: 10.1136/vr.140.1.13.
Thirteen isolates of Cowdria ruminantium were made from eight different Districts of Kenya by four different isolation methods. Feeding adult Amblyomma species ticks derived from nymphs collected in the field and the inoculation of homogenates prepared from adult field ticks had the highest success rate. The reattachment of adult ticks collected in the field was successful on only one of five attempts, and the subinoculation of blood from suspected heartwater carriers was unsuccessful. Seven of the isolates were derived from A variegatum ticks, four from A gemma, one from A lepidum and one from a mixed pool of the last two species. This is the first report of the isolation of C ruminantium from A gemma ticks, and the first report of its transtadial transmission from nymphal to adult A gemma.
通过四种不同的分离方法，从肯尼亚八个不同地区分离出了13株反刍兽考德里氏体。用采自野外若虫培育出的成年钝缘蜱属蜱虫进行喂养，以及接种由野外成年蜱虫制备的匀浆，成功率最高。野外采集的成年蜱虫重新附着，五次尝试中只有一次成功，而从疑似心水病携带者采集血液进行传代接种未成功。其中7株分离株源自变异革蜱，4株源自宝石革蜱，1株源自鳞盾革蜱，1株源自后两种蜱虫的混合样本。这是首次从宝石革蜱中分离出反刍兽考德里氏体的报告，也是首次关于其在宝石革蜱若虫到成虫期间经变态传递的报告。
