Davranov K, Diiarov Zh, Rizaeva M
Prikl Biokhim Mikrobiol. 1978 May-Jun;14(3):389-98.
Lipase biosynthesis by the fungus Rhizopus microsporus UzLT-1 was investigated. Under certain conditions the fungus could produce two forms of extracellular lipases. The paper describes the process of purification of the enzyme L-I,N- and C-terminal amino acids of the lipases have been determined. Identity of terminal amino acids of both enzymes has been demonstrated. It has been shown that both forms of lipase from Rhizopus microporus hydrolyze predominantly alpha-ester bonds.
对微小根毛霉UzLT-1合成脂肪酶的过程进行了研究。在特定条件下,该真菌可产生两种形式的胞外脂肪酶。本文描述了脂肪酶L-I的纯化过程,已测定了其N端和C端氨基酸序列。两种酶的末端氨基酸序列具有一致性。研究表明,微小根毛霉的两种脂肪酶主要水解α-酯键。