Popov V L, Kirillova F M, Orlova O E
Zh Mikrobiol Epidemiol Immunobiol. 1984 May(5):30-3.
The surface structure of chlamydial elementary bodies (strain PL-B577 causing enzootic abortion of ewes and strain CP-1 isolated from a patient with Reiter's syndrome), concentrated directly on the grid of an electron microscope, was studied by the method of negative staining. The surface of the cell wall of chlamydial elementary bodies in the preparations of crude cultures, not subjected to enzymatic purification, was formed by spherical subunits 4 nm in diameter. The surface of the cell wall of elementary bodies was found to have projections 10-20 nm long and 3-4 nm in diameter, as well as annular structures 15-22 nm in diameter in hexagonal arrangement, spaced at 45 nm and probably traversed by these projections. The number of such structures in a group was 12-15. These data correspond to the model representing the structure of the surface of chlamydiae, which was proposed by Matsumoto in 1979.
采用负染色法,对直接浓缩在电子显微镜载网上的衣原体原体(引起母羊地方性流产的PL - B577菌株和从赖特综合征患者分离出的CP - 1菌株)的表面结构进行了研究。在未经酶纯化的粗培养物制剂中,衣原体原体细胞壁表面由直径4nm的球形亚基构成。发现原体细胞壁表面有长10 - 20nm、直径3 - 4nm的突起,以及直径15 - 22nm呈六边形排列、间距为45nm且可能被这些突起穿过的环形结构。一组中此类结构的数量为12 - 15个。这些数据与松本在1979年提出的衣原体表面结构模型相符。