鸡早期发育和遗传性营养不良时骨骼肌中的糖鞘脂

Glycosphingolipids of chicken skeletal muscle in early development and genetic dystrophy.

作者信息

Hogan E L, Chien J L, Dasgupta S

出版信息

Adv Exp Med Biol. 1984;174:287-96. doi: 10.1007/978-1-4684-1200-0_24.

Abstract

The acidic and neutral GSL of chicken pectoral muscle and the activities of relevant sialyltransferase and glycosidases have been examined during embryonic and early post-hatching development. At this stage of myogenesis, a prominent shift to the neutral GSL of longer oligosaccharide length involving Forssman glycolipid most prominently and also globoside and GbOse3Cer occurred but the distribution of muscle-type gangliosides was not obviously affected. The glycosidase and sialyltransferase activities decreased dramatically just prior to or at hatching. The fusion-linked change in GSL suggests a role for terminal galactosamine and/or galactose residues in myoblast aggregation. A parallel developmental study of genetic muscular dystrophy revealed similar GSL levels and enzyme activities. A larger proportion of lactosylceramide in dystrophic muscle throughout development suggests a developmental lag in the mutant.

摘要

在胚胎期和孵化后早期发育阶段，对鸡胸肌的酸性和中性糖鞘脂以及相关唾液酸转移酶和糖苷酶的活性进行了检测。在这个肌生成阶段，出现了向中性糖鞘脂的显著转变，其中寡糖长度更长，最显著的是福斯曼糖脂，还有球蛋白和GbOse3Cer，但肌肉型神经节苷脂的分布没有受到明显影响。糖苷酶和唾液酸转移酶的活性在孵化前或孵化时急剧下降。糖鞘脂中与融合相关的变化表明末端半乳糖胺和/或半乳糖残基在成肌细胞聚集中发挥作用。对遗传性肌肉萎缩症的平行发育研究揭示了相似的糖鞘脂水平和酶活性。在整个发育过程中，营养不良肌肉中乳糖基神经酰胺的比例更大，这表明突变体存在发育滞后现象。