Schauer R, Schröder C, Shukla A K
Adv Exp Med Biol. 1984;174:75-86. doi: 10.1007/978-1-4684-1200-0_7.
Sialic acid analysis in biological material including gangliosides is often confronted with the necessity to determine trace amounts of various N,O-substituted species. Therefore, techniques of high sensitivity and resolution are required, such as capillary gas-liquid chromatography (GLC) and high performance liquid chromatography (HPLC). Both methods in combination with mass spectrometry allow structural analysis of the different neuraminic acid derivatives. Thus, the number of natural sialic acids known so far has increased to more than 30, including non only saturated, but also 2,3-unsaturated and 2,7-anhydro-sialic acids. Furthermore, HPLC has proved to be especially useful for the study of enzyme reactions, as the sialic acids of enzyme assay mixtures in most cases can be analyzed without prior extensive purification or derivatization.
对包括神经节苷脂在内的生物材料中的唾液酸进行分析时，常常需要测定痕量的各种N、O取代的唾液酸种类。因此，需要高灵敏度和高分辨率的技术，如毛细管气液色谱法（GLC）和高效液相色谱法（HPLC）。这两种方法与质谱联用可对不同的神经氨酸衍生物进行结构分析。因此，迄今已知的天然唾液酸数量已增加到30多种，不仅包括饱和唾液酸，还包括2,3-不饱和唾液酸和2,7-脱水唾液酸。此外，HPLC已被证明对酶反应的研究特别有用，因为在大多数情况下，酶分析混合物中的唾液酸无需事先进行广泛的纯化或衍生化即可进行分析。
