Barajas L, Powers K, Wang P
Am J Physiol. 1984 Jul;247(1 Pt 2):F50-60. doi: 10.1152/ajprenal.1984.247.1.F50.
A quantitative assessment of the innervation of the different portions of the cortical tubular nephron in the rat was made using tritiated norepinephrine uptake to label monoaminergic nerves, followed by autoradiography. The proximal tubules (PT) showed the greatest number of innervated profiles (IPs) [i.e., tubular profiles overlapped by accumulations of autoradiographic grains (AAG)] followed by the thick ascending limb of Henle (TALH), the distal convoluted tubule (DCT), and the collecting duct (CD). However, the highest relative frequency of innervation (RFI), i.e., the ratio of the number of IPs to the total number of profiles surveyed, occurred in the TALH followed by the DCT and PT. The number of IPs of the CDs was too low to permit adequate statistical evaluation. The PT showed the largest number of AAGs facing the interstitium or in contact with capillaries, while the TALH had the highest number in contact with the efferent arteriole. The DCT showed a disproportionately high number of AAGs in contact with the afferent arteriole. Our results suggest that all portions of the cortical tubular nephron are under some degree of neural influence.
利用氚标记的去甲肾上腺素摄取来标记单胺能神经，随后进行放射自显影，对大鼠皮质肾小管不同部分的神经支配进行了定量评估。近端小管（PT）显示出受神经支配的轮廓（IPs）数量最多[即，被放射自显影颗粒（AAG）聚集重叠的肾小管轮廓]，其次是髓袢升支粗段（TALH）、远曲小管（DCT）和集合管（CD）。然而，神经支配相对频率（RFI）最高，即IPs数量与所调查轮廓总数的比值，出现在TALH，其次是DCT和PT。CD的IPs数量过低，无法进行充分的统计学评估。PT显示出面向间质或与毛细血管接触的AAG数量最多，而TALH与出球小动脉接触的AAG数量最多。DCT显示与入球小动脉接触的AAG数量不成比例地高。我们的结果表明，皮质肾小管的所有部分都受到一定程度的神经影响。
