McFeeters R F, Armstrong S A
Anal Biochem. 1984 May 15;139(1):212-7. doi: 10.1016/0003-2697(84)90407-x.
A procedure was developed to measure the degree of pectin methylation in small samples of isolated cell walls from nonlignified plant tissues or pectin solutions. Galacturonic acid was determined colorimetrically with the 3,5-dimethylphenol reagent. Methylation was measured by base hydrolysis of galacturonic acid methyl esters, followed by gas chromatographic determination of released methanol. Estimates of the precision of analysis of pectin and cell wall samples were made. The coefficient of variation for estimates of the pectin esterification in cell walls isolated from 10-g samples of cucumber tissue ranged from 7.7 to 13.2%.
已开发出一种程序，用于测量来自非木质化植物组织的分离细胞壁小样本或果胶溶液中果胶甲基化程度。用3,5 - 二甲基苯酚试剂比色法测定半乳糖醛酸。通过半乳糖醛酸甲酯的碱水解，然后气相色谱法测定释放的甲醇来测量甲基化。对果胶和细胞壁样本的分析精度进行了估计。从10克黄瓜组织样本中分离出的细胞壁中果胶酯化估计值的变异系数在7.7%至13.2%之间。
