连续酶反应的动力学。偶联测定法的设计及途径的时间响应。
The kinetics of consecutive enzyme reactions. The design of coupled assays and the temporal response of pathways.
作者信息
Easterby J S
出版信息
Biochem J. 1984 May 1;219(3):843-7. doi: 10.1042/bj2190843.
A regime is proposed for the design of coupled enzyme assays in which auxiliary enzymes are added at concentrations proportional to their Km values. Under these conditions it is possible to calculate the complete time course of the assay including the time required for the system to approach its steady state. The consequence of increasing the number of coupling enzymes is shown to be a considerable decrease in time required to reach the steady state provided that the overall transient time remains the same. The method is extended to the general consideration of pathways and shows that pathways of the same length exhibit identical temporal responses provided that the units of concentration and time used are based on the steady-state concentration of intermediates and the transient time respectively. An unexpected finding is that increasing the number of intermediates in a pathway can decrease the time required to enter a steady state.
本文提出了一种用于设计偶联酶测定的方案，其中辅助酶的添加浓度与其Km值成正比。在这些条件下，可以计算测定的完整时间进程，包括系统达到稳态所需的时间。结果表明，增加偶联酶的数量会使达到稳态所需的时间显著减少，前提是总瞬态时间保持不变。该方法被扩展到对途径的一般考虑，并表明相同长度的途径表现出相同的时间响应，前提是所使用的浓度和时间单位分别基于中间体的稳态浓度和瞬态时间。一个意外的发现是，增加途径中中间体的数量可以减少进入稳态所需的时间。
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