Lagtime：一个用于计算偶联酶分析参数的程序。
Lagtime: a program for calculating coupled enzyme assay parameters.
作者信息
Brooks S P, Suelter C H
出版信息
Int J Biomed Comput. 1986 Sep;19(2):101-7. doi: 10.1016/0020-7101(86)90004-8.
A program is described for calculating either (i) the time required for the observed rate to approximate the rate of the enzyme under study (the lagtime) when the concentrations of the auxiliary enzymes are known or (ii) the units of auxiliary enzymes needed to obtain a desired lagtime. The calculations for these coupled enzyme systems also apply to cases where one of the intermediates undergoes mutarotation; for example, equations for coupled reactions involving two enantiomers of glucose as intermediates incorporate the rate constants for mutarotation. When two auxiliary enzymes are used, the program also minimizes the total cost of the assay if the price per unit of the coupling enzymes are known. The equations used are those of S.P.J. Brooks et al. (Can. J. Biochem. Cell Biol. 62 (1984) 945-955; 956-963.
本文描述了一个程序，用于计算：(i) 当辅助酶浓度已知时，观察到的速率接近所研究酶的速率所需的时间（滞后时间）；或 (ii) 获得所需滞后时间所需的辅助酶单位数。这些偶联酶系统的计算也适用于其中一种中间体发生变旋的情况；例如，涉及葡萄糖两种对映体作为中间体的偶联反应方程纳入了变旋速率常数。当使用两种辅助酶时，如果已知偶联酶的单价，该程序还能使测定的总成本最小化。所使用的方程是S.P.J.布鲁克斯等人的方程（《加拿大生物化学与细胞生物学杂志》62 (1984) 945 - 955；956 - 963）。
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