刀豆脲酶中的镍离子环境。
The nickel ion environment in jack bean urease.
作者信息
Alagna L, Hasnain S S, Piggott B, Williams D J
出版信息
Biochem J. 1984 Jun 1;220(2):591-5. doi: 10.1042/bj2200591.
Abstract
Preliminary results of an extended X-ray absorption fine structure (e.x.a.f.s.) and X-ray absorption near edge structure study of jack bean urease have recently been reported [Hasnain & Piggott (1983) Biochem. Biophys. Res. Commun. 112, 279]. These results indicate that the environment of the nickel ion in the enzyme is similar to that in the model compounds Ni(L)2(L')1(ClO4)1 (where L is 1-n-propyl-2-alpha-hydroxybenzylbenzimidazole and L' is the deprotonated form) and Ni(HMB)3(Br)2 (where HMB is 2-hydroxymethylbenzimidazole), the closest similarity being with Ni(L)2-(L')1(ClO4)1. A detailed e.x.a.f.s. analysis has now been carried out and the crystal structures of the two model compounds solved. These results are reported here.
摘要
刀豆脲酶的扩展X射线吸收精细结构（EXAFS）和X射线吸收近边结构研究的初步结果最近已有报道[哈斯奈因和皮戈特（1983年），《生物化学与生物物理研究通讯》，第112卷，第279页]。这些结果表明，酶中镍离子的环境与模型化合物Ni(L)2(L')1(ClO4)1（其中L为1-正丙基-2-α-羟基苄基苯并咪唑，L'为去质子化形式）和Ni(HMB)3(Br)2（其中HMB为2-羟甲基苯并咪唑）中的环境相似，与Ni(L)2-(L')1(ClO4)1最为相似。现已进行了详细的EXAFS分析，并解析了这两种模型化合物的晶体结构。此处报告这些结果。
相似文献
1
The nickel ion environment in jack bean urease.刀豆脲酶中的镍离子环境。
Biochem J. 1984 Jun 1;220(2):591-5. doi: 10.1042/bj2200591.
2
An EXAFS study of jack bean urease, a nickel metalloenzyme.
Biochem Biophys Res Commun. 1983 Apr 15;112(1):279-83. doi: 10.1016/0006-291x(83)91827-2.
3
Jack bean urease (EC 3.5.1.5). I. A simple dry ashing procedure for the microdetermination of trace metals in proteins. The nickel content of urease.刀豆脲酶（EC 3.5.1.5）。I. 一种用于蛋白质中痕量金属微量测定的简单干灰化程序。脲酶的镍含量。
Can J Biochem. 1980 Jun;58(6):469-73. doi: 10.1139/o80-062.
4
Jack been urease (EC 3.5.1.5). II. The relationship between nickel, enzymatic activity, and the "abnormal" ultraviolet spectrum. The nickel content of jack beans.杰克豆中的脲酶（EC 3.5.1.5）。二、镍、酶活性与“异常”紫外光谱之间的关系。杰克豆中的镍含量。
Can J Biochem. 1980 Jun;58(6):474-80. doi: 10.1139/o80-063.
5
Jack bean urease (EC 3.5.1.5). III. The involvement of active-site nickel ion in inhibition by beta-mercaptoethanol, phosphoramidate, and fluoride.杰克豆脲酶（EC 3.5.1.5）。III. 活性位点镍离子在β-巯基乙醇、氨基磷酸酯和氟化物抑制作用中的参与情况。
Can J Biochem. 1980 Jun;58(6):481-8. doi: 10.1139/o80-064.
6
Crystallization and preliminary X-ray structure determination of jack bean urease with a bound antibody fragment.结合抗体片段的刀豆脲酶的结晶及初步X射线晶体结构测定
Acta Crystallogr D Biol Crystallogr. 2002 Feb;58(Pt 2):374-6. doi: 10.1107/s0907444901021503. Epub 2002 Jan 24.
7
Structural change of jack bean urease induced by addition of surfactants studied with synchrotron-radiation small-angle X-ray scattering.
Eur J Biochem. 1993 Jul 1;215(1):55-61. doi: 10.1111/j.1432-1033.1993.tb18006.x.
8
The structure of jack bean urease. The complete amino acid sequence, limited proteolysis and reactive cysteine residues.刀豆脲酶的结构。完整氨基酸序列、有限蛋白酶解及反应性半胱氨酸残基
Eur J Biochem. 1988 Jul 15;175(1):151-65. doi: 10.1111/j.1432-1033.1988.tb14177.x.
9
Cloning and sequencing of a jack bean urease-encoding cDNA.
Gene. 1991 Dec 15;108(2):265-7. doi: 10.1016/0378-1119(91)90443-f.
10
A procedure for purifying jack bean urease for clinical use.一种用于临床用途的刀豆脲酶纯化方法。
Proc Natl Sci Counc Repub China B. 1989 Oct;13(4):250-7.
引用本文的文献
1
Urea Decomposition Mechanism by Dinuclear Nickel Complexes.双核镍配合物的尿素分解机理。
Molecules. 2023 Feb 9;28(4):1659. doi: 10.3390/molecules28041659.
2
Ligand-Modulated Nuclearity and Geometry in Nickel(II) Hydrazone Complexes: From Mononuclear Complexes to Acetato- and/or Phenoxido-Bridged Clusters.镍(II)腙配合物中配体调节的核数和几何构型：从单核配合物到乙酰氧/或苯氧桥联簇。
Int J Mol Sci. 2023 Jan 18;24(3):1909. doi: 10.3390/ijms24031909.
3
The structure-based reaction mechanism of urease, a nickel dependent enzyme: tale of a long debate.脲酶的基于结构的反应机制，一种镍依赖性酶：漫长争论的故事。
J Biol Inorg Chem. 2020 Sep;25(6):829-845. doi: 10.1007/s00775-020-01808-w. Epub 2020 Aug 18.
4
Nonredox nickel enzymes.非氧化还原镍酶
Chem Rev. 2014 Apr 23;114(8):4206-28. doi: 10.1021/cr4004488. Epub 2013 Dec 26.
5
The involvement of amino acid side chains in shielding the nickel coordination site: an NMR study.氨基酸侧链在屏蔽镍配位位中的作用：NMR 研究。
Molecules. 2013 Oct 8;18(10):12396-414. doi: 10.3390/molecules181012396.
6
Diazido-bis[(1-methyl-1H-benzimidazol-2-yl)methanol-κN,O]manganese(II).二叠氮基双[（1-甲基-1H-苯并咪唑-2-基）甲醇-κN，O]锰（II）
Acta Crystallogr Sect E Struct Rep Online. 2010 Jan 23;66(Pt 2):m188. doi: 10.1107/S1600536810002126.
7
Interplay of metal ions and urease.金属离子与脲酶的相互作用。
Metallomics. 2009;1(3):207-21. doi: 10.1039/b903311d.
8
Expression of catalytically active recombinant Helicobacter pylori urease at wild-type levels in Escherichia coli.在大肠杆菌中以野生型水平表达具有催化活性的重组幽门螺杆菌脲酶。
Infect Immun. 1993 Jun;61(6):2563-9. doi: 10.1128/iai.61.6.2563-2569.1993.
9
An extended-X-ray-absorption-fine-structure (e.x.a.f.s.) study of coenzyme F430 from Methanobacterium thermoautotrophicum.嗜热自养甲烷杆菌辅酶F430的扩展X射线吸收精细结构（EXAFS）研究
Biochem J. 1985 Nov 15;232(1):281-4. doi: 10.1042/bj2320281.
10
Cloning of urease gene sequences from Providencia stuartii.从斯氏普罗威登斯菌中克隆脲酶基因序列。
Infect Immun. 1986 Oct;54(1):161-9. doi: 10.1128/iai.54.1.161-169.1986.
本文引用的文献
1
Stereochemistry of iron in deoxyhaemoglobin.
Nature. 1982 Feb 11;295(5849):535-8. doi: 10.1038/295535a0.
2
An EXAFS study of jack bean urease, a nickel metalloenzyme.
Biochem Biophys Res Commun. 1983 Apr 15;112(1):279-83. doi: 10.1016/0006-291x(83)91827-2.
3
Jack bean urease (EC 3.5.1.5). V. On the mechanism of action of urease on urea, formamide, acetamide, N-methylurea, and related compounds.刀豆脲酶（EC 3.5.1.5）。五、脲酶对尿素、甲酰胺、乙酰胺、N-甲基脲及相关化合物的作用机制
Can J Biochem. 1980 Dec;58(12):1335-44. doi: 10.1139/o80-181.
4
Jack bean urease (EC 3.5.1.5). Demonstration of a carbamoyl-transfer reaction and inhibition by hydroxamic acids.刀豆脲酶（EC 3.5.1.5）。氨基甲酰基转移反应的证明及异羟肟酸的抑制作用。
Biochemistry. 1969 May;8(5):1991-2000. doi: 10.1021/bi00833a032.
5
Letter: Jack bean urease (EC 3.5.1.5). A metalloenzyme. A simple biological role for nickel?
J Am Chem Soc. 1975 Jul 9;97(14):4131-3. doi: 10.1021/ja00847a045.
Zotero 插件