血清白蛋白可增强培养的人成纤维细胞从磷脂酰胆碱囊泡摄取[3H]胆固醇的能力。
Serum albumin enhances the uptake of [3H]cholesterol from phosphatidylcholine vesicles by cultured human fibroblasts.
作者信息
Slotte J P, Björkerud S
出版信息
Biochem J. 1984 Jun 1;220(2):605-8. doi: 10.1042/bj2200605.
Abstract
Cultured human lung fibroblasts, incubated with cholesterol/phosphatidylcholine vesicles (cholesterol: phosphatidylcholine molar ratio 1:1) incorporated vesicle [3H]-cholesterol linearly for at least 48 h by an exchange process without gaining sterol mass. The incorporation of [3H]cholesterol by the cells was markedly enhanced in the presence of purified bovine serum albumin. A fraction of the incorporated vesicle [3H]cholesterol was esterified by the cells.
摘要
培养的人肺成纤维细胞与胆固醇/磷脂酰胆碱囊泡（胆固醇：磷脂酰胆碱摩尔比为1:1）一起孵育，通过交换过程线性摄取囊泡[3H] - 胆固醇至少48小时，且没有获得固醇质量。在纯化的牛血清白蛋白存在下，细胞对[3H]胆固醇的摄取显著增强。细胞将一部分摄取的囊泡[3H]胆固醇酯化。
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本文引用的文献
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J Biol Chem. 1982 May 10;257(9):4775-82.
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6
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