Pincus S, Rekosh D
Biochem Biophys Res Commun. 1984 Jun 29;121(3):1033-41. doi: 10.1016/0006-291x(84)90781-2.
The effect of actinomycin D on adenovirus DNA replication has been examined both in vivo and in a cell-free extract capable of replication on exogenously added template. In both cases we show that 5 micrograms/ml of drug cause an inhibition of DNA synthesis of at least 80%. The in vitro results further demonstrate that both DNA chain growth (elongation) and initiation - the addition of the first nucleotide of the DNA chain (dCMP) to the preterminal protein - are inhibited directly by the drug, by not by alpha-amanitin.
放线菌素D对腺病毒DNA复制的影响已在体内以及一种能够在外源添加模板上进行复制的无细胞提取物中进行了研究。在这两种情况下，我们都表明5微克/毫升的药物会导致至少80%的DNA合成受到抑制。体外实验结果进一步表明，DNA链的生长（延伸）和起始——即DNA链的第一个核苷酸（dCMP）添加到末端前体蛋白上——均直接受到该药物的抑制，而非α-鹅膏蕈碱的抑制。
