Pinckard R N, Jackson E M, Hoppens C, Weintraub S T, Ludwig J C, McManus L M, Mott G E
Biochem Biophys Res Commun. 1984 Jul 18;122(1):325-32. doi: 10.1016/0006-291x(84)90478-9.
The molecular heterogeneity of platelet-activating factor (PAF) produced by stimulated human neutrophilic polymorphonuclear leukocytes (PMN) was assessed by both normal and reverse phase high performance liquid chromatography (HPLC). As detected by rabbit platelet stimulation, at least 5 PAF molecules were separated by HPLC. Fast atom bombardment (FAB) mass spectrometry revealed one of these PAFs was acetyl glyceryl ether phosphorylcholine (AGEPC) with a C16:0 alkyl chain in the sn-1 position. Although the structures of the remaining PAFs are unknown, two of the peaks of PAF activity had the same retention times on reverse phase HPLC as the C15- and C18-saturated alkyl chain AGEPC homologues. These studies indicate that the human PMN produces multiple molecular species of PAF.
通过正相和反相高效液相色谱法（HPLC）评估了受刺激的人嗜中性多形核白细胞（PMN）产生的血小板活化因子（PAF）的分子异质性。通过兔血小板刺激检测到，HPLC分离出至少5种PAF分子。快原子轰击（FAB）质谱分析表明，其中一种PAF是在sn-1位带有C16:0烷基链的乙酰甘油醚磷酸胆碱（AGEPC）。尽管其余PAF的结构尚不清楚，但PAF活性的两个峰在反相HPLC上的保留时间与C15和C18饱和烷基链AGEPC同系物相同。这些研究表明人PMN产生多种分子形式的PAF。
