Kuijpers G A, Van Nooy I G, De Pont J J, Bonting S L
Biochim Biophys Acta. 1984 Jul 25;774(2):269-76. doi: 10.1016/0005-2736(84)90301-8.
The isolated rabbit pancreas secretes a fluid containing chloride and bicarbonate in about equal concentrations. Replacement of bicarbonate by acetate, phosphate or isethionate, replacement of Na+ by Li+ and addition of ouabain to the bathing medium of the pancreas inhibit the secretion of fluid, chloride and bicarbonate in a similar fashion and by maximally 100%. Replacement of chloride by isethionate inhibits fluid secretion by maximally 50%, chloride secretion by 90% and bicarbonate secretion by 20%. It is concluded that fluid secretion is based on a Na+-gradient-dependent bicarbonate influx or proton efflux in the ductular cell, and that the secretion of chloride is secondary to that of bicarbonate.
分离出的兔胰腺分泌一种含有浓度大致相等的氯离子和碳酸氢根离子的液体。用乙酸盐、磷酸盐或羟乙磺酸盐替代碳酸氢根离子，用锂离子替代钠离子，以及在胰腺的灌流液中添加哇巴因，会以类似方式并最大程度地抑制100%的液体、氯离子和碳酸氢根离子的分泌。用羟乙磺酸盐替代氯离子会最大程度地抑制50%的液体分泌、90%的氯离子分泌和20%的碳酸氢根离子分泌。得出的结论是，液体分泌基于导管细胞中依赖钠离子梯度的碳酸氢根离子内流或质子外流，并且氯离子的分泌继发于碳酸氢根离子的分泌。
