利用共振拉曼光谱研究类胡萝卜素在脂质双层中的取向有序性。
Study of the orientational ordering of carotenoids in lipid bilayers by resonance-Raman spectroscopy.
作者信息
van de Ven M, Kattenberg M, van Ginkel G, Levine Y K
出版信息
Biophys J. 1984 Jun;45(6):1203-9. doi: 10.1016/S0006-3495(84)84269-1.
The orientational ordering of beta-carotene and crocetin embedded in lamellar model membranes has been investigated by angle-resolved resonance Raman scattering at a temperature well above the phase transition of the lipid chains. It is shown that the ordering of the carotenoids is dependent on the chemical composition of the lipid bilayers. The orientational distribution functions found clearly show that beta-carotene is oriented parallel to the bilayer plane (dioleoyl lecithin) or perpendicular to it (soybean lecithin). For dimyristoyl lecithin at 40 degrees C, egg-lecithin, and digalactosyl diacylglycerol two maxima were found in the orientational distribution: one parallel and one perpendicular to the bilayer surface. Crocetin embedded in soybean lecithin bilayers yields a similar bimodal distribution function. Because of rapid photodegradation no results could be obtained for spirilloxanthin.
在远高于脂质链相变温度的条件下，通过角分辨共振拉曼散射研究了嵌入层状模型膜中的β-胡萝卜素和藏红花素的取向有序性。结果表明，类胡萝卜素的有序性取决于脂质双层的化学组成。所发现的取向分布函数清楚地表明，β-胡萝卜素平行于双层平面（二油酰卵磷脂）或垂直于双层平面（大豆卵磷脂）取向。对于40℃的二肉豆蔻酰卵磷脂、蛋黄卵磷脂和二半乳糖二酰基甘油，在取向分布中发现了两个最大值：一个平行于双层表面，一个垂直于双层表面。嵌入大豆卵磷脂双层中的藏红花素产生了类似的双峰分布函数。由于快速光降解，无法获得螺旋藻黄素的结果。
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