Hearse D J
Cardiovasc Res. 1984 Jun;18(6):384-90. doi: 10.1093/cvr/18.6.384.
A new procedure is described which allows for the rapid homogenisation, extraction and analysis of the metabolite content of microbiopsy samples (milligram quantities) while completely overcoming the major errors arising as the consequence of the substantial and variable tissue loss associated with conventional procedures. In addition to allowing more accurate and faster analysis of much smaller quantities of tissue the procedure also allows for the coincident paired measurement of flow (radioactive microspheres) in each biopsy. An example of the application of the method to the measurement of flow and high energy phosphate content in multiple microbiopsy samples from normal and ischaemic canine myocardium is provided.
本文描述了一种新方法，该方法可对微量活检样本（毫克级）的代谢物含量进行快速匀浆、提取和分析，同时完全克服了因传统方法导致的大量且可变的组织损失而产生的主要误差。除了能够对更少量的组织进行更准确、快速的分析外，该方法还允许对每个活检样本中的血流（放射性微球）进行同步配对测量。文中给出了该方法应用于测量正常和缺血犬心肌多个微量活检样本中的血流和高能磷酸盐含量的示例。
