[14C]苯乙烯在离体大鼠肝细胞中的DNA结合。

DNA binding of [14C]styrene in isolated rat hepatocytes.

作者信息

Legraverend C, Elovaara E, Zitting A, Heinonen T, Uusvuori R

出版信息

Chem Biol Interact. 1984 Sep 1;51(1):91-102. doi: 10.1016/0009-2797(84)90022-x.

Abstract

Incubation of hepatocytes from phenobarbital-pretreated rats with diethylmaleate (DEM) and 1 mM [14C]styrene for 3 to 5 hours did not result in binding of styrene 7,8-oxide (SO) to DNA as determined by ion exchange chromatography of enzymatic digests of DNA. The elution of substantial amounts of radioactivity together with natural nucleosides and bases suggests that styrene is partly metabolized via splitting of the vinyl bond and that incorporation of C1 fragments into DNA is most likely the result of repair DNA synthesis following DNA damage by styrene itself or one of its metabolites.

摘要

用马来酸二乙酯（DEM）和1 mM [¹⁴C]苯乙烯对经苯巴比妥预处理的大鼠的肝细胞进行孵育3至5小时，通过对DNA酶解产物进行离子交换色谱分析，未发现苯乙烯7,8 - 氧化物（SO）与DNA结合。大量放射性与天然核苷和碱基一起洗脱，这表明苯乙烯部分通过乙烯基键的断裂进行代谢，并且C1片段掺入DNA很可能是苯乙烯本身或其代谢产物对DNA造成损伤后修复性DNA合成的结果。