Marvin W J, Chittick V L, Rosenthal J K, Sandra A, Atkins D L, Hermsmeyer K
Circ Res. 1984 Aug;55(2):253-60. doi: 10.1161/01.res.55.2.253.
We prepared primary cell cultures of the sinus node region from newborn rat hearts. Sinoatrial node cells were easily distinguished from the other cardiac muscle cells and nonmuscle cells in culture by size, configuration, and rapid, attenuated spontaneous contractions (185.0 +/- 8/min, mean +/- SEM). The spontaneously contracting sinoatrial node cells were extremely sensitive to acetylcholine and norepinephrine, responding to concentrations at least 1000-fold less than other cardiac muscle cells. These same sinoatrial node cells in culture were fixed and precisely relocated by either subsequent scanning or transmission electron microscopy. The ultrastructural features of these sinoatrial node cells in culture were similar to those observed in the cells of intact sinus node sections from the source hearts. This study is the first to present single, spontaneously active, neonatal sinoatrial node cells maintained in vitro with morphological and functional properties desirable for physiological investigations.
我们从新生大鼠心脏制备了窦房结区域的原代细胞培养物。在培养过程中，窦房结细胞很容易通过大小、形态以及快速、衰减的自发收缩（185.0±8/分钟，平均值±标准误）与其他心肌细胞和非肌肉细胞区分开来。自发收缩的窦房结细胞对乙酰胆碱和去甲肾上腺素极其敏感，其反应浓度至少比其他心肌细胞低1000倍。培养中的这些相同窦房结细胞通过后续的扫描或透射电子显微镜进行固定和精确重新定位。培养中这些窦房结细胞的超微结构特征与从来源心脏完整窦房结切片的细胞中观察到的特征相似。本研究首次展示了体外培养的单个、自发活跃的新生窦房结细胞，其具有生理研究所需的形态和功能特性。
