Mineral and endocrine metabolism during fracture healing in dogs.

The blood levels of parathormone (PTH), calcitonin (CT), vitamin D metabolites, calcium, phosphate, magnesium and alkaline phosphatase (AKP) were determined in 13 young dogs with experimental fractures of long bones. The parameters were measured before the fracture and during healing until the appearance of an early callus. Serum Ca levels were significantly reduced immediately after the fracture (10.95 +/- 0.30 mg/dl to 10.25 +/- 0.25 mg/dl; p less than 0.05). Significant rises in serum levels of PTH (0.99 +/- 0.10 ng/ml to 1.57 +/- 0.22 ng/ml; p less than 0.01); CT (0.14 +/- 0.01 ng/ml to 0.18 +/- 0.01 ng/ml; p less than 0.05); 24,25-dihydroxyvitamin D (13 +/- 2 ng/ml to 19 +/- 3 ng/ml; p less than 0.01) and AKP (153 +/- 24 U/l to 211 +/- 26 U/l; p less than 0.01) were noted during fracture repair. A reduction in serum levels of 25-hydroxyvitamin D (22 +/- 2 ng/ml to 15 +/- 1 ng/ml; p less than 0.001) was noted at the same time. The parameters returned or tended to return to normal levels during callus development. The data demonstrate the changes in levels of Ca-regulating hormones in response to bone injury. This suggests that CT and 24,25-dihydroxyvitamin D (24,25-(OH)2-D) can be associated with the process of callus formation.