Bonete M J, Manjon A, Llorca F, Iborra J L
Comp Biochem Physiol B. 1984;78(1):203-6. doi: 10.1016/0305-0491(84)90169-x.
Acid catheptic activity was measured in crude extracts of muscle, liver, heart, spleen and gonads from the fishes Mujil auratus, Sparus aurata and Lightonatus mormyrus. The spleen was the organ which showed the highest activity. A comparative study of the seven most commonly used extraction methods was made. Some were modified to account for the characteristics of the fish organs and the activity extracted from them. The Siebert method resulted as the best extraction method only if 1 mM EDTA was present in the medium. The activity from Mujil auratus muscle was strongly inhibited by iodoacetate, N-ethylmaleimide, p-hydroxy mercuribenzoate, and diazo-acetyl-DL-norleucine methyl ester. The results indicated the presence of a carboxyl-proteinase and a thiol-proteinase. According to inhibition studies, the levels of proteinase and amidase activities shown by different organs of Mujil auratus were re-examined. The spleen extract showed the maximum activity for both cathepsins, but muscle extract accounted for more than 95% of total catheptic activity.
测定了金头鲷、黑鲷和光齿鲷的肌肉、肝脏、心脏、脾脏和性腺粗提物中的酸性组织蛋白酶活性。脾脏是活性最高的器官。对七种最常用的提取方法进行了比较研究。对其中一些方法进行了修改，以适应鱼类器官的特性以及从中提取的活性。仅当培养基中存在1 mM乙二胺四乙酸（EDTA）时，西伯特法才是最佳提取方法。碘乙酸、N-乙基马来酰亚胺、对羟基汞苯甲酸和重氮乙酰-DL-正亮氨酸甲酯对金头鲷肌肉的活性有强烈抑制作用。结果表明存在一种羧基蛋白酶和一种巯基蛋白酶。根据抑制研究，重新检查了金头鲷不同器官的蛋白酶和酰胺酶活性水平。脾脏提取物对两种组织蛋白酶均显示出最大活性，但肌肉提取物占总组织蛋白酶活性的95%以上。
