Danielpour D, Ikeda T, Kunkel M W, Sirbasku D A
Endocrinology. 1984 Sep;115(3):1221-3. doi: 10.1210/endo-115-3-1221.
An acid- and heat-stable low molecular weight (Mr 2,500-6,200) autostimulatory growth factor has been identified in extracts of GH3/C14 rat pituitary tumors, in extracts of these cells in culture and in their serum-free conditioned medium. The concentrations of autostimulatory activity required to achieve ED50 in serum-free culture were 4.3, 4.0 and 85 micrograms/ml for extracts of tumors, the cultured cells, and from the conditioned medium of the cells in culture, respectively. To characterize this activity, we have used an isolation method that included 0.1 M acetic acid extraction, heating at 95 C, SP-Sephadex chromatography, and finally, Sephadex G-50 chromatography. The identification of this activity if rapidly growing, relatively autonomous GH3/C14 tumors suggests an autostimulatory role in the conversion from estrogen-responsive growth to autonomy.
在GH3/C14大鼠垂体肿瘤提取物、这些细胞的培养提取物及其无血清条件培养基中，已鉴定出一种耐酸且耐热的低分子量（Mr 2,500 - 6,200）自刺激生长因子。在无血清培养中达到ED50所需的自刺激活性浓度，肿瘤提取物、培养细胞提取物和细胞培养条件培养基分别为4.3、4.0和85微克/毫升。为了表征这种活性，我们采用了一种分离方法，包括0.1 M乙酸提取、95℃加热、SP - 葡聚糖凝胶色谱，最后是葡聚糖凝胶G - 50色谱。在快速生长、相对自主的GH3/C14肿瘤中鉴定出这种活性，表明其在从雌激素反应性生长向自主性转变中具有自刺激作用。
