Szaniawska B, Majewski S, Kamiński M J, Noremberg K, Swierz M, Janik P
J Natl Cancer Inst. 1985 Aug;75(2):303-6.
Lung-conditioned medium (LCM) was obtained by incubation of BALB/c mouse lung tissue fragments in serum-free Eagle medium for 48 hours and subsequent separation by dialysis or chromatography on a Sephadex G-75 column. LCM fractions were tested for their ability to modulate proliferation of normal human endothelial cells and neoplastic cells (N2a, MCF, HEp-2) in vitro as assessed by plating efficiency and tritiated thymidine incorporation assays. It was found that LCM contained two kinds of factors, either stimulating (molecular weight: 50,000-70,000) or inhibiting (molecular weight: 12,000-20,000 and 3,000-5,000) cell proliferation.
肺条件培养基（LCM）通过将BALB/c小鼠肺组织碎片在无血清伊格尔培养基中孵育48小时，随后通过透析或在Sephadex G-75柱上进行色谱分离获得。通过平板接种效率和氚标记胸腺嘧啶核苷掺入试验评估，测试了LCM组分在体外调节正常人内皮细胞和肿瘤细胞（N2a、MCF、HEp-2）增殖的能力。结果发现，LCM含有两种因子，一种刺激细胞增殖（分子量：50,000 - 70,000），另一种抑制细胞增殖（分子量：12,000 - 20,000和3,000 - 5,000）。
