Nseyo U O, Englander L S, Huben R P, Pontes J E
Fertil Steril. 1984 Aug;42(2):281-4.
The most important method for evaluating the pathogenesis of male infertility is open testicular biopsy. Herein we describe a method of aspiration biopsy of testis for histologic examination. Sexually mature dogs and rats treated with chemotherapeutic agents and ionizing radiation were followed with periodic testicular aspiration biopsy during and after treatment. The histologic findings from the aspiration biopsy compare with the results of routine histologic examinations in assessing spermatogenetic activity and delineating pathologic changes. The puncture in the experimental animals was performed under general anesthesia. In human patients testicular biopsy could be done under local anesthesia in an outpatient clinic. The procedure would be less painful, minimally invasive, and more cost-effective.
评估男性不育症发病机制的最重要方法是开放性睾丸活检。在此，我们描述一种用于组织学检查的睾丸穿刺活检方法。对接受化疗药物和电离辐射治疗的性成熟犬和大鼠在治疗期间及治疗后进行定期睾丸穿刺活检随访。穿刺活检的组织学结果与常规组织学检查结果在评估生精活性和描绘病理变化方面进行比较。实验动物的穿刺在全身麻醉下进行。在人类患者中，睾丸活检可在门诊局部麻醉下进行。该操作疼痛较轻、创伤极小且更具成本效益。
