Feurle G E, Winkler C, Encke A, Helmstaedter V, Doertenbach J G
Digestion. 1984;29(3):183-9. doi: 10.1159/000199029.
In order to study some of the molecular events during the hepatic passage of gastrin, we perfused sulfated natural porcine gastrin (G17 II) through isolated pig livers. The disappearance half time of G17 II was about 20-30 min when the starting gastrin concentrations were greater than 100 pM; lower concentrations were reduced with half times of 40-100 min. Synthetic human leu-32 (G34) was not eliminated. The use of region-specific antibodies to gastrin indicated that degradation was more effective at the N-terminus of gastrin. Whereas Sephadex chromatography revealed no change of the molecular size, SDS-polyacrylamide gel electrophoresis showed the presence of smaller immunoreactive fragments of gastrin in addition to immunoreactive fragments of gastrin of the heptadecapeptide size. These findings indicate that the isolated porcine liver degrades porcine G17 to smaller fragments.
为了研究胃泌素在肝脏转运过程中的一些分子事件，我们通过离体猪肝灌注硫酸化天然猪胃泌素（G17 II）。当起始胃泌素浓度大于100 pM时，G17 II的消失半衰期约为20 - 30分钟；较低浓度时半衰期延长至40 - 100分钟。合成人亮氨酸-32（G34）未被清除。使用针对胃泌素的区域特异性抗体表明，胃泌素在其N端的降解更有效。虽然葡聚糖凝胶色谱显示分子大小没有变化，但SDS-聚丙烯酰胺凝胶电泳显示除了十七肽大小的胃泌素免疫反应性片段外，还存在较小的胃泌素免疫反应性片段。这些发现表明，离体猪肝可将猪G17降解为较小的片段。
