Serum enzyme determinations in the diagnosis of acute myocardial infarction: an update.

Serum CK-MB and LD-1 have proved extremely useful in the diagnosis and differential diagnosis of acute myocardial infarction. However, CK-MB is present in skeletal muscle and can be released during ischemic attacks; thus, abnormal serum CK-MB activities cannot be equated with myocardial injury. Even wider is the distribution of LD-1, which is found particularly in erythrocytes and renal cortex; hence, an abnormal LD-1 level also cannot be equated with myocardial injury. The method of choice and the final arbiter for the CK and LD isoenzymes is electrophoresis. The possibility of interpreting the results visually fulfills, in part, quality-control needs, and makes the technique suitable for small and large laboratories. Extreme analytic sensitivity is not needed, and electrophoresis provides clinically useful and acceptable results.