Appell H J
Int J Sports Med. 1984 Jun;5(3):125-9. doi: 10.1055/s-2008-1025893.
The effects of muscular activity on the motor end-plates were studied. To simulate an overload training on the diaphragm, Japanese waltzer mice were subjected to hypobaric hypoxia (simulated altitude of 3000 m) for 7 and 14 days. After demonstration of the end-plates by AChE-staining, their size and total number in the sternal part of the diaphragm were measured at the light microscopic level. The end-plate regions were enlarged after 7 days and were increased in number after 14 days. These changes can probably be explained by the mechanisms of terminal and collateral sprouting. The electron-microscopic examination showed no ultrastructural peculiarities in either of the experimental groups. The muscle fibers of the trained animals showed a strong reaction of AChE; thus, it was suggested that end-plate proliferation was myogenically induced.
研究了肌肉活动对运动终板的影响。为模拟膈肌的超负荷训练，将日本华尔兹小鼠置于低压低氧环境（模拟海拔3000米）中7天和14天。通过乙酰胆碱酯酶染色显示终板后，在光学显微镜水平测量膈肌胸骨部终板的大小和总数。7天后终板区域增大，14天后终板数量增加。这些变化可能可以用终末和侧支发芽机制来解释。电子显微镜检查显示两个实验组均无超微结构异常。训练动物的肌纤维显示出强烈的乙酰胆碱酯酶反应；因此，提示终板增殖是由肌源性诱导的。
