Olson K J, Fontenot J P, Failla M L
J Anim Sci. 1984 Jul;59(1):210-6. doi: 10.2527/jas1984.591210x.
Thirty-six mature, nongestating, crossbred ewes were allotted randomly to three diets for 140 d: basal (six ewes); 50% basal and 50% broiler litter, dry basis (24 ewes) and 50% basal and 50% deep stacked broiler litter with the addition of 25 ppm molybdenum (Mo) and 5 g sulfate (SO4)/kg feed (six ewes). The control diet and the broiler litter contained 8 and 257 ppm Cu, dry basis, respectively. Liver samples from six ewes in each group were obtained by biopsy initially and at 40, 80 and 120 d and at slaughter (140 d). The remaining 18 litter-fed ewes were fed the basal diet after 140 d and six were slaughtered after 30-, 60- and 120-d. Liver Cu content continually increased (P less than .01) by feeding broiler litter (404 vs 1,543 ppm, dry basis), and was decreased (962 ppm) by supplementing Mo and SO4. During the withdrawal period, liver and duodenum Cu levels were not reduced (P greater than .05). A linear increase (P less than .001) in serum glutamic oxalacetic transaminase (SGOT) was observed with time during the withdrawal period. Serum Cu decreased at 30-d withdrawal and increased thereafter (quadratic effect (P less than .05). Hepatic Cu-metallothionein levels increased 15-fold above control values in the litter-fed ewes (P less than .05).
36只成年、非妊娠的杂交母羊被随机分为三组，分别饲喂三种日粮，为期140天：基础日粮组（6只母羊）；50%基础日粮和50%干基肉鸡垫料组（24只母羊）；50%基础日粮和50%深层堆积肉鸡垫料并添加25 ppm钼（Mo）和5 g硫酸盐（SO4）/kg饲料组（6只母羊）。对照日粮和肉鸡垫料的干基铜含量分别为8 ppm和257 ppm。每组6只母羊的肝脏样本最初在第40、80和120天以及屠宰时（第140天）通过活检获取。其余18只采食垫料的母羊在140天后改喂基础日粮，其中6只分别在30天、60天和120天后屠宰。饲喂肉鸡垫料可使肝脏铜含量持续增加（P < 0.01）（干基：404 vs 1543 ppm），而添加钼和硫酸盐则使其降低（962 ppm）。在撤料期，肝脏和十二指肠的铜水平未降低（P > 0.05）。撤料期血清谷氨酸草酰乙酸转氨酶（SGOT）随时间呈线性增加（P < 0.001）。血清铜在撤料30天时降低，之后升高（二次效应，P < 0.05）。采食垫料的母羊肝脏铜金属硫蛋白水平比对照值增加了15倍（P < 0.05）。
