用高效液相色谱法分析高密度脂蛋白中的载脂蛋白

Analysis of apolipoproteins in high density lipoproteins by high performance liquid chromatography.

作者信息

Kinoshita M, Okazaki M, Kato H, Teramoto T, Matsushima T, Naito C, Oka H, Hara I

出版信息

J Biochem. 1984 Apr;95(4):1111-8. doi: 10.1093/oxfordjournals.jbchem.a134699.

DOI:10.1093/oxfordjournals.jbchem.a134699

Abstract

A simple and rapid method for the analysis of apolipoproteins in high density lipoprotein (HDL) by high performance liquid chromatography (HPLC) has been developed (Kinoshita et al. (1983) J. Biochem. 94, 615-617). With this method, using a sodium phosphate buffer containing 0.1% sodium dodecyl sulfate (SDS) as an eluent, apolipoproteins can be analyzed from a very small amount of HDL fraction without delipidation using organic solvents. Separation profiles of apolipoproteins by this method were examined using several techniques. The elution pattern monitored by A280 can give precise quantitative as well as qualitative information about size-distribution of apolipoproteins, except for the apo C group. Moreover, separation of apo E from apo A-I was found to be improved by column elongation.

摘要

已开发出一种通过高效液相色谱法（HPLC）分析高密度脂蛋白（HDL）中载脂蛋白的简单快速方法（Kinoshita等人，（1983年）《生物化学杂志》94卷，615 - 617页）。使用该方法，以含有0.1%十二烷基硫酸钠（SDS）的磷酸钠缓冲液作为洗脱剂，无需使用有机溶剂进行脱脂，就能从极少量的HDL组分中分析载脂蛋白。通过几种技术对该方法分离载脂蛋白的图谱进行了研究。除载脂蛋白C组外，通过280nm吸光度监测的洗脱模式可提供有关载脂蛋白大小分布的精确定量和定性信息。此外，发现通过延长柱长可改善载脂蛋白E与载脂蛋白A - I的分离效果。