Lanfranchi G, Linder S, Ringertz N R
J Cell Sci. 1984 Mar;66:309-19. doi: 10.1242/jcs.66.1.309.
Chick globin synthesis was studied in heterokaryons formed between chick erythrocytes and human K562 erythroleukemia cells or rat L6J1 myoblasts. It was found that chick globin synthesis was activated after fusion of definitive (17 to 19-day) chick erythrocytes obtained from 17 to 19-day embryos with K562 cells. Chick globins appeared to be of the adult alpha A, alpha D and beta types, whereas no embryonic globin synthesis could be detected. The pattern and time-course of globin synthesis was investigated after fusion of 4 to 5-day embryonic erythrocytes with rat L6J1 myoblasts. The level of globin synthesis was high at early time points but then decreased. Globin synthesis, however, was still detectable at 9 days in these heterokaryons. Chick alpha A, alpha D and epsilon-globin chain synthesis was observed both at early and late time points after fusion.
研究了鸡珠蛋白在鸡红细胞与人类K562红白血病细胞或大鼠L6J1成肌细胞形成的异核体中的合成情况。发现从17至19天胚胎获得的定型（17至19天）鸡红细胞与K562细胞融合后，鸡珠蛋白的合成被激活。鸡珠蛋白似乎是成年型的αA、αD和β型，而未检测到胚胎珠蛋白的合成。在4至5天的胚胎红细胞与大鼠L6J1成肌细胞融合后，研究了珠蛋白合成的模式和时间进程。珠蛋白合成水平在早期时间点较高，但随后下降。然而，在这些异核体中，9天时仍可检测到珠蛋白合成。在融合后的早期和晚期时间点均观察到鸡αA、αD和ε-珠蛋白链的合成。
