Vaillier J, Vaillier D
Rev Can Biol. 1978 Mar;37(1):27-34.
The hydrophobic fluorescent probe, 1-anilino-8-naphthalene sulphonate (ANS), has been used to study conformational changes of mouse antigen-stimulated lymphocytes in vivo. Studies revealed that early conformational changes appear in Bovine Serum Albumin-- or Sheep Erythrocytes-- stimulated splenic and thymic lymphocytes. These conformational changes are detected by fluorescence intensity changes, when ANS is bound to lymphocytes. The kinetic studies further indicate that the course of conformational changes may vary considerably depending on antigens.
疏水荧光探针1-苯胺基-8-萘磺酸盐（ANS）已被用于研究小鼠体内抗原刺激淋巴细胞的构象变化。研究表明，在牛血清白蛋白或绵羊红细胞刺激的脾细胞和胸腺细胞中会出现早期构象变化。当ANS与淋巴细胞结合时，可通过荧光强度变化检测到这些构象变化。动力学研究进一步表明，构象变化的过程可能因抗原的不同而有很大差异。
