Slack J M
J Embryol Exp Morphol. 1984 Apr;80:321-30.
The development of ectoderm isolated from the animal pole of axolotl gastrulae is monitored by light microscopy, electron microscopy and analysis of newly synthesized proteins, glycoproteins and glycolipids. When control embryos are undergoing neurulation it is shown that the explants autonomously begin to express epidermal markers and do not express mesodermal markers. However the results suggest that not all the cells become epidermal and electron microscope examination shows that only the outer layer does so, the inner cells remaining undifferentiated.
通过光学显微镜、电子显微镜以及对新合成的蛋白质、糖蛋白和糖脂的分析，监测从蝾螈原肠胚动物极分离出的外胚层的发育情况。当对照胚胎进行神经胚形成时，结果显示外植体自主开始表达表皮标记物，而不表达中胚层标记物。然而，结果表明并非所有细胞都变成表皮细胞，电子显微镜检查显示只有外层细胞如此，内部细胞仍未分化。
