Sadoughi M
Acta Morphol Neerl Scand. 1981 Jun;19(2):127-34.
Presumptive ectoderm from young gastrulae of the Pleurodele and the Axolotl was treated with concanavalin A(5-25 micrograms/ml). Then, it was combined, in a sandwich, with the dorsal lip of the blastopore and cultured in vitro for 5-7 days. The results of the experiments show that the treated ectoderm becomes more fragile than untreated control ectoderm. Nevertheless, the neural induction and the histological differentiation of the neural tissue appeared in a rather normal fashion.
用伴刀豆球蛋白A(5 - 25微克/毫升)处理有肋螈和蝾螈幼原肠胚的推定外胚层。然后,将其与胚孔背唇以夹心方式组合,并在体外培养5 - 7天。实验结果表明,经处理的外胚层比未处理的对照外胚层更脆弱。然而,神经诱导和神经组织的组织学分化以相当正常的方式出现。