6-羟基多巴胺孵育对大鼠肛门尾骨肌去甲肾上腺素蓄积及收缩反应的影响。

Effect of 6-hydroxydopamine incubation on noradrenaline accumulation and on contractile responses in the rat anococcygeus muscle.

作者信息

Doggrell S A, Woodruff G N

出版信息

Res Commun Chem Pathol Pharmacol. 1978 May;20(2):253-64.

Abstract

The contractile responses of the rat anococcygeus muscle to sympathomimetics, dopaminergic agonists and 5-hydroxytryptamine, but not to acetylcholine, were inhibited by phentolamine (10(-5)M). Following 6-hydroxydopamine incubation (10(-3)M for 3h), the accumulation of noradrenaline was inhibited, contractile responses to noradrenaline were potentiated, responses to tyramine were abolished and responses to acetylcholine were unaltered. Thus, any spasmogen activity due to release of noradrenaline in this muscle was abolished by 6-hydroxydopamine incubation. This method was used to characterise the direct and indirect actions of some sympathomimetics. In addition, dopaminergic agonists and 5-hydroxytryptamine may also act by releasing noradrenaline in this preparation.

摘要

大鼠肛门尾骨肌对拟交感神经药、多巴胺能激动剂和5-羟色胺的收缩反应（但对乙酰胆碱无收缩反应）可被酚妥拉明（10⁻⁵M）抑制。在6-羟基多巴胺孵育（10⁻³M，3小时）后，去甲肾上腺素的蓄积受到抑制，对去甲肾上腺素的收缩反应增强，对酪胺的反应消失，对乙酰胆碱的反应未改变。因此，通过6-羟基多巴胺孵育可消除该肌肉中因去甲肾上腺素释放而产生的任何致痉活性。此方法用于表征某些拟交感神经药的直接和间接作用。此外，多巴胺能激动剂和5-羟色胺在该制剂中也可能通过释放去甲肾上腺素发挥作用。