Shimizu K, Yaguchi S, Kometani A, Nakajyo S, Ozaki H, Urakawa N
Jpn J Pharmacol. 1984 Feb;34(2):231-9. doi: 10.1254/jjp.34.231.
In the present paper, a comparative study of high K solution was carried out on the mechanical response, wet and dry weights of tissue, and extracellular space of 14C-sorbitol in the trachea, gall bladder, and urinary bladder of guinea-pig. Hyperosmotic addition of KCl to the physiological solution produced a sustained contraction, while isosmotic substitution of K for Na in the physiological solution induced a contraction followed by a small sustained one in the trachea, gall bladder or urinary bladder. At 120 min after application of the substituted KCl, Na deficient solution, it increased cell water content in the smooth muscle cells of the trachea or gall bladder, but did not change that in the urinary bladder. Both the inhibition of contraction and the swelling in the trachea or gall bladder were prevented by the hyperosmotic application of sucrose or NaCl. The substitution for NaCl with the K salts with more permeable anions (I- greater than NO3- greater than Cl-) produced a greater inhibition of contraction in the trachea and gall bladder. Although the impermeable anion C2H5CO2- did not inhibit the contraction in the trachea, this anion partially inhibited it in the gall bladder. On the other hand, the inhibition of contraction in the urinary bladder was prevented by addition of pyruvate, oxalacetate or NaCl.(ABSTRACT TRUNCATED AT 250 WORDS)
在本论文中，对高钾溶液在豚鼠气管、胆囊和膀胱的机械反应、组织湿重与干重以及14C-山梨醇细胞外间隙方面进行了比较研究。向生理溶液中高渗添加氯化钾会产生持续收缩，而在生理溶液中用钾等渗替代钠会在气管、胆囊或膀胱中诱发一次收缩，随后是一次小的持续收缩。在应用替代氯化钾、缺钠溶液120分钟后，它增加了气管或胆囊平滑肌细胞中的细胞含水量，但未改变膀胱中的细胞含水量。气管或胆囊中收缩的抑制和肿胀可通过高渗应用蔗糖或氯化钠来防止。用具有更高通透性阴离子（碘离子大于硝酸根离子大于氯离子）的钾盐替代氯化钠，在气管和胆囊中对收缩产生更大的抑制作用。尽管不可通透阴离子乙酸根离子在气管中不抑制收缩，但该阴离子在胆囊中部分抑制收缩。另一方面，通过添加丙酮酸、草酰乙酸或氯化钠可防止膀胱中的收缩受到抑制。（摘要截短于250字）
