Schaub R G, Simmons C A, Koets M H, Romano P J, Stewart G J
Lab Invest. 1984 Aug;51(2):218-24.
A jugular vein was exposed in 20 cats divided into four groups of five cats each. In group 1 the vein was removed immediately after exposure. In group 2 the vein was removed after three 5-minute periods of stasis and reflow. Groups 3 and 4 had the jugular vein occluded for 24 and 72 hours, respectively. In all groups, veins were perfused under physiologic pressure by heparinized saline to remove blood and immersed in 2.5% glutaraldehyde for fixation. All vessels were prepared for scanning and transmission electron microscopy. Group 1 cats had a normal-appearing luminal surface. Group 2 cats had deposition of leukocytes with few erythrocytes or platelets. Groups 3 and 4 had deposition of leukocytes, platelets, and erythrocytes. Leukocytes were found in all areas and associated with all cell types. Platelets and erythrocytes were seldom found in the absence of leukocytes. Thrombi were found on normal-appearing and damaged endothelium. The majority of thrombi were found at side branches and valve pockets. Our results suggest that leukocytes play a primary role in the initiation of deep vein thrombosis. Platelets may have only a secondary role.
在20只猫身上暴露颈静脉，将其分为四组，每组五只猫。在第1组中，静脉暴露后立即切除。在第2组中，静脉在经历三个5分钟的血流停滞和再灌注周期后切除。第3组和第4组的颈静脉分别闭塞24小时和72小时。在所有组中，通过肝素化盐水在生理压力下灌注静脉以清除血液，并浸入2.5%的戊二醛中进行固定。所有血管均制备用于扫描电子显微镜和透射电子显微镜检查。第1组猫的管腔表面外观正常。第2组猫有白细胞沉积，红细胞或血小板很少。第3组和第4组有白细胞、血小板和红细胞沉积。在所有区域均发现白细胞，且与所有细胞类型相关。在没有白细胞的情况下很少发现血小板和红细胞。在外观正常和受损的内皮上均发现血栓。大多数血栓见于侧支和瓣膜袋处。我们的结果表明，白细胞在深静脉血栓形成的起始中起主要作用。血小板可能仅起次要作用。
