Baron D A, Briggman J V, Spicer S S
Lab Invest. 1984 Aug;51(2):233-43.
Human eccrine sweat glands, including those expressing the gene for cystic fibrosis, were stained sequentially en bloc with uranyl acetate, lead citrate and copper sulfate, and osmium tetroxide for the ultrastructural demonstration of tubulocisternal endoplasmic reticulum (TER). This organelle has been associated with water and electrolyte transport in other epithelia. TER was present in the apical cytoplasm and basolateral cytoplasm circumscribing intercellular canaliculi of clear cells in the secretory coil and in the apical cuticular cytoplasm of luminal cells in the coiled duct. In both the secretory coil and duct, TER was situated strategically around the tight junctions. Together, the TER and tight junctions may regulate the secretory and absorptive permeability of the eccrine sweat gland to NaCl and water. The dark cell of the coil which is exocrine, and the deep cell of the duct which lacked tight junctions, did not contain TER. In sweat glands obtained from three cases of cystic fibrosis, elements of TER were more prevalent and complex in luminal cells of the secretory duct than was the TER in normal sweat glands. This difference may be a consequence of the basic electrolyte abnormality of cystic fibrosis.
人外泌汗腺，包括那些表达囊性纤维化基因的汗腺，依次用醋酸铀、柠檬酸铅和硫酸铜以及四氧化锇进行整体染色，以超微结构显示管状-池状内质网（TER）。这种细胞器在其他上皮细胞中与水和电解质运输有关。TER存在于分泌盘曲部透明细胞的细胞间小管周围的顶端细胞质和基底外侧细胞质中，以及蟠曲导管腔面细胞的顶端角质细胞质中。在分泌盘曲部和导管中，TER都位于紧密连接周围。TER和紧密连接共同作用，可能调节外泌汗腺对氯化钠和水的分泌及吸收通透性。盘曲部的外分泌暗细胞和缺乏紧密连接的导管深层细胞不含TER。在取自3例囊性纤维化患者的汗腺中，分泌导管腔面细胞中的TER成分比正常汗腺中的TER更普遍、更复杂。这种差异可能是囊性纤维化基本电解质异常的结果。
