MUNGER B L
J Biophys Biochem Cytol. 1961 Nov;11(2):385-402. doi: 10.1083/jcb.11.2.385.
The electron microscopy of human eccrine sweat glands has been studied before and after stimulation by pilocarpine iontophoresis. The identity of the dark and clear cells in the secretory segment as defined by Montagna et al. (23) was determined by studying serial sections, thin for electron microscopy and thick for light microscopy. Cells with numerous apical secretory vacuoles are termed mucoid (dark) cells, since these vacuoles stain positively for acid mucopolysaccharide. Clear cells are intimately associated with intercellular canaliculi. The "cuticular border" of surface cells of the duct is a condensation of tonofilaments and granules. Numerous mitochondria are concentrated in basal cells of the duct. The presence of mucoid cells in the secretory segment may bear on the interpretation of the pathologic findings in the disease cystic fibrosis of the pancreas, and suggests that this disease may be due to a basic disorder of mucopolysaccharide production. The possible roles of the various cellular components in the elaboration of sweat are discussed.
对毛果芸香碱离子导入刺激前后的人外泌汗腺进行了电子显微镜研究。通过研究连续切片（薄切片用于电子显微镜观察，厚切片用于光学显微镜观察），确定了蒙塔尼亚等人（23）所定义的分泌段中暗细胞和明细胞的特征。具有大量顶端分泌泡的细胞被称为黏液样（暗）细胞，因为这些泡对酸性黏多糖呈阳性染色。明细胞与细胞间小管密切相关。导管表面细胞的“角质缘”是张力丝和颗粒的凝聚物。大量线粒体集中在导管的基底细胞中。分泌段中黏液样细胞的存在可能与胰腺囊性纤维化疾病的病理结果解释有关，并表明该疾病可能是由于黏多糖产生的基本紊乱所致。讨论了各种细胞成分在汗液分泌过程中的可能作用。
