Dupret J, Grenot C, Rolland de Ravel M, Mappus E, Cuilleron C Y
J Steroid Biochem. 1984 Jun;20(6A):1345-52. doi: 10.1016/0022-4731(84)90167-5.
Anti-testosterone (T) and anti-5 alpha-dihydrotestosterone (DHT) antibodies were raised in rabbits after preimmunization with 17 beta-hemisuccinamido, 7 beta-hemiglutaramido- and 3 beta-hemiglutaramido haptens of DHT and T covalently linked to D-glutamic acid-D-lysine (D-GL) copolymer, followed by immunization with the corresponding haptens covalently linked to bovine serum albumin. Preimmunization with DHT-D-GL in the case of anti-T-antibodies or with T-D-GL, in the case of anti-DHT antibodies significantly lowered the T-DHT cross-reactivity in all cases, the most striking effect being observed with anti-17 beta-hemisuccinamido-T antibodies (CR less than 1%). The evolution with time of the binding characteristics was also studied, showing that in several cases the lowered T-DHT cross-reactivity could be maintained after several booster injections until a useful titer was reached. The better results obtained with 17 beta-hemisuccinamido haptens suggest that the structure of the hapten exerts a strong influence on the induction of immunotolerance.
用与D - 谷氨酸 - D - 赖氨酸（D - GL）共聚物共价连接的双氢睾酮（DHT）和睾酮（T）的17β - 半琥珀酰胺基、7β - 半谷氨酰胺基和3β - 半谷氨酰胺基半抗原对家兔进行预免疫，然后用与牛血清白蛋白共价连接的相应半抗原进行免疫，从而产生抗睾酮（T）和抗双氢睾酮（DHT）抗体。在抗T抗体的情况下用DHT - D - GL进行预免疫，或在抗DHT抗体的情况下用T - D - GL进行预免疫，在所有情况下均显著降低了T - DHT交叉反应性，在抗17β - 半琥珀酰胺基 - T抗体中观察到最显著的效果（交叉反应率小于1%）。还研究了结合特性随时间的演变，结果表明在几种情况下，经过几次加强注射后，降低的T - DHT交叉反应性可以维持，直至达到有用的效价。用17β - 半琥珀酰胺基半抗原获得的更好结果表明，半抗原的结构对免疫耐受性的诱导有很大影响。
