Pampori N A, Singh G, Srivastava V M
Mol Biochem Parasitol. 1984 Apr;11:205-13. doi: 10.1016/0166-6851(84)90066-5.
Incorporation of 32Pi into organic phosphate by mitochondria of Cotugnia digonopora was supported maximally by malate. Fumarate and succinate induced lower but significant production of ATP. Pyruvate, alpha-ketoglutarate and oxalacetate proved to be poor substrates and citrate and isocitrate had no effect. A net phosphorylation of approximately 2 mol of ADP was observed for each mol of CO2 liberated from malate or succinate. In contrast, with pyruvate, in spite of a high rate of decarboxylation, the production of ATP was extremely low. 2,4-Dinitrophenol inhibited phosphorylation. All anthelmintics examined interfered with the mitochondrial phosphorylation of ADP, with maximum inhibition by salicylanilide compounds. The anticestodal activity of the latter group of compounds, niclosamide for example, may, therefore, be attributed to their ability to inhibit mitochondrial phosphorylation.
双睾阔盘吸虫线粒体将³²Pi掺入有机磷酸盐的过程中，苹果酸对其支持作用最大。富马酸和琥珀酸诱导产生的ATP量较低，但仍很显著。丙酮酸、α-酮戊二酸和草酰乙酸被证明是较差的底物，而柠檬酸和异柠檬酸则没有作用。每从苹果酸或琥珀酸释放1摩尔CO₂，可观察到约2摩尔ADP的净磷酸化。相比之下，丙酮酸尽管脱羧速率很高，但ATP的产生却极低。2,4-二硝基苯酚抑制磷酸化。所有检测的驱虫药都干扰了ADP的线粒体磷酸化，水杨酰苯胺类化合物的抑制作用最大。因此，后一组化合物（例如氯硝柳胺）的抗绦虫活性可能归因于它们抑制线粒体磷酸化的能力。
