Taylor R F, Davies B H
Can J Biochem. 1982 Jun;60(6):675-83. doi: 10.1139/o82-083.
A cell-free enzyme extract from Streptococcus faecium UNH 564P has been prepared. The extract incorporates either [2-14C]mevalonic acid (MVA) or [1-14C]isopentenyl pyrophosphate (IPP) into squalene and the carotenoids of the bacterium. ATP and manganese ion were found to be absolute requirements for MVA incorporation by the extract. Only manganese ion was found to be an absolute requirement for IPP incorporation by the extract. Other cofactors including magnesium ion, glutathione, potassium fluoride, NADP, and FAD significantly increase the incorporation of both substrates into the S. faecium terpenoids. Isolation and purification of the radioactive terpenoids from the cell-free system confirmed that the carotenoids of S. faecium are triterpenoids.
已制备出粪肠球菌UNH 564P的无细胞酶提取物。该提取物可将[2-¹⁴C]甲羟戊酸（MVA）或[1-¹⁴C]异戊烯基焦磷酸（IPP）掺入该细菌的角鲨烯和类胡萝卜素中。发现ATP和锰离子是提取物将MVA掺入所绝对必需的。仅发现锰离子是提取物将IPP掺入所绝对必需的。包括镁离子、谷胱甘肽、氟化钾、NADP和FAD在内的其他辅因子可显著增加两种底物掺入粪肠球菌萜类化合物中的量。从无细胞体系中分离并纯化放射性萜类化合物证实，粪肠球菌的类胡萝卜素是三萜类化合物。
