Bandman E, Matsuda R, Strohman R C
Cell. 1982 Jun;29(2):645-50. doi: 10.1016/0092-8674(82)90180-5.
Myosin heavy chains prepared from the pectoralis major and from the posterior latissimus dorsi of the same adult chicken exhibit different peptide maps when cleaved with Staphylococcus aureus V8 protease. These differences were observed at five different enzyme concentrations and in chickens of various strains. The cleavage pattern of pectoralis major myosin heavy chain from different adult chickens was always identical, as was that of posterior latissimus dorsi myosin heavy chain, demonstrating the reproducibility of the technique. However, when RNAs extracted from the pectoralis major and from the posterior latissimus dorsi were translated in a cell-free reticulocyte lysate, the myosin heavy chain encoded by pectoralis major RNA and the myosin heavy chain encoded by posterior latissimus dorsi RNA exhibited identical peptide maps. These results suggest that the different peptide maps of myosin heavy chains from the pectoralis major and posterior latissimus dorsi may arise from posttranslational modifications.
从同一只成年鸡的胸大肌和背阔肌后部制备的肌球蛋白重链，在用金黄色葡萄球菌V8蛋白酶切割时，呈现出不同的肽图。在五种不同的酶浓度下以及在各种品系的鸡中均观察到了这些差异。来自不同成年鸡的胸大肌肌球蛋白重链的切割模式始终相同，背阔肌后部肌球蛋白重链的切割模式也是如此，这证明了该技术的可重复性。然而，当从胸大肌和背阔肌后部提取的RNA在无细胞网织红细胞裂解物中进行翻译时，由胸大肌RNA编码的肌球蛋白重链和由背阔肌后部RNA编码的肌球蛋白重链呈现出相同的肽图。这些结果表明，胸大肌和背阔肌后部的肌球蛋白重链的不同肽图可能源于翻译后修饰。
