Shahin M M, Bugaut A, Junino A, Kalopissis G
Carcinogenesis. 1982;3(7):809-13. doi: 10.1093/carcin/3.7.809.
Two samples of 4-amino-2-nitrophenol were tested for mutagenicity in Salmonella typhimurium strains TA1535, TA1537, TA1538, TA98, and TA100. Significant mutagenic activity was detected with a sample of this compound obtained from Aldrich (technical grade (Aldrich), TGA) both in the presence and in the absence of a rat-liver S9 metabolic activation mixture. When toluene-insoluble contaminants of the TGA product were separated and tested for mutagenicity, they were found to be mutagenic in the same strains as the TGA sample. After removal of these major contaminants from the TGA product its mutagenicity was reduced; a nonmutagenic sample was obtained by further purification. The other sample of 4-amino-2-nitrophenol, which was synthesized and purified in our laboratory, was nonmutagenic. H.p.l.c. profiles of the unpurified TGA sample and the purified samples revealed a large number of contaminants in the TGA product. Likewise, t.l.c. revealed the presence of contaminants in the TGA product.
对两个4-氨基-2-硝基苯酚样品进行了鼠伤寒沙门氏菌TA1535、TA1537、TA1538、TA98和TA100菌株的致突变性测试。从奥德里奇公司获得的该化合物样品（技术级（奥德里奇），TGA），无论在有或没有大鼠肝脏S9代谢活化混合物存在的情况下，均检测到显著的致突变活性。当分离出TGA产品中不溶于甲苯的污染物并测试其致突变性时，发现它们在与TGA样品相同的菌株中具有致突变性。从TGA产品中去除这些主要污染物后，其致突变性降低；通过进一步纯化获得了一个无致突变性的样品。在我们实验室合成并纯化的另一个4-氨基-2-硝基苯酚样品无致突变性。未纯化的TGA样品和纯化样品的高效液相色谱图显示TGA产品中有大量污染物。同样，薄层层析显示TGA产品中存在污染物。
