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用于微量滴定板孔微观扫描的索引阶段。

An indexing stage for microscopic scanning of microtitre tray wells.

作者信息

Darling W, Justin O, Pike B L, Jennings G, Shortman K

出版信息

J Immunol Methods. 1982;52(1):39-42. doi: 10.1016/0022-1759(82)90347-7.

Abstract

A simple stage has been designed to hold and to move microtitre trays for examination under a low power dissecting microscope. Movement of a ball on a handle from well to well of a reference tray to the left of the stage is mechanically translated into movement from one well to another under the microscope field. Movement can be controlled entirely by touch, and the particular well under the field can be determined from the reference tray position. The flat bottoms of all 96 wells stay in alignment and in focus without further adjustment, enabling rapid scanning of all wells on a tray. The apparatus is particularly useful for the microtitre tray antibody-forming cell plaque assay described by Pike et al. (1982).

摘要

已设计出一种简易载物台,用于放置和移动微量滴定板,以便在低倍解剖显微镜下进行检查。载物台左侧有一个参考板,通过手柄上的球在参考板各孔之间移动,这种移动会机械转换为显微镜视野下从一个孔到另一个孔的移动。移动完全可以通过触摸控制,并且视野下的特定孔可根据参考板的位置确定。所有96孔的平底无需进一步调整即可保持对齐和聚焦,从而能够快速扫描板上的所有孔。该装置对于派克等人(1982年)描述的微量滴定板抗体形成细胞空斑测定特别有用。

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