McGregor D B, McConville M, Menzies C, Prentice R D
Mutat Res. 1982 Jul-Aug;102(1):39-50. doi: 10.1016/0165-1218(82)90144-6.
The mutagenicity of 2-acetylaminofluorene (AAF) in S. typhimurium TA 1538 was investigated using Ames' test and activation systems based upon rat- or cotton rat-liver post-mitochondrial supernatant (S9) fractions. Part of this study involved sub-fractionation of S9 into microsomes (M) and 100,000 X g supernatant (S100) fractions. With a rat liver-derived fractions, mos activity was associated with S100; M-activating potential was never greater than that achieved with S9. In cotton rats, most activating potential was associated with S9. This activity was greater than could be accounted for by the separated cotton-rat M and S100 components. Reconstituted, cross-species 'S9' fraction studies showed that the dominant determinant of S9 properties was the M fraction in both rats and cotton rats. The principal co-factor required in the activation reactions was NADPH, but it could be largely replaced by NADH. 7,8-Benzoflavone inhibited activation both in M and S100 whereas paraoxon had no effect upon rat S100 activation, but had a marked effect upon cotton-rat M activation.
利用艾姆斯试验及基于大鼠或棉鼠肝脏线粒体后上清液（S9）组分的活化系统，研究了2-乙酰氨基芴（AAF）在鼠伤寒沙门氏菌TA 1538中的诱变性。本研究的一部分涉及将S9亚分级为微粒体（M）和100,000×g上清液（S100）组分。对于大鼠肝脏来源的组分，大部分活性与S100相关；M的活化潜力从未超过S9的活化潜力。在棉鼠中，大部分活化潜力与S9相关。这种活性大于棉鼠分离的M和S100组分所能解释的活性。重组的跨物种“S9”组分研究表明，大鼠和棉鼠中S9性质的主要决定因素都是M组分。活化反应所需的主要辅助因子是NADPH，但它在很大程度上可被NADH替代。7,8-苯并黄酮抑制M和S100中的活化，而对氧磷对大鼠S100活化无影响，但对棉鼠M活化有显著影响。
