Bignami M, Carere A, Conti G, Conti L, Crebelli R, Fabrizi M
Mutat Res. 1982 Aug;97(4):293-302. doi: 10.1016/0165-1161(82)90028-0.
21 chemicals, known to induce missense and/or frameshift mutations directly, were assayed for their ability to forward mutate a haploid strain of A. nidulans. 2 genetic markers for forward mutations were used, namely 8-azaguanine resistance and induction of meth A1 suppressors. Missense mutagens were usually active when tested with the plate-incorporation technique, whereas frameshift agents were ineffective; some of these, on the other hand, turned out to be positive when tested with a liquid-test procedure. The 2 genetic markers used showed a similar sensitivity (with only 2 exceptions) in detecting the chemical mutagens assayed.
对已知能直接诱导错义突变和/或移码突变的21种化学物质进行了检测，以评估它们使构巢曲霉单倍体菌株发生正向突变的能力。使用了2个正向突变的遗传标记，即对8-氮鸟嘌呤的抗性和甲硫氨酸A1抑制子的诱导。当用平板掺入技术检测时，错义诱变剂通常具有活性，而移码诱变剂则无效；另一方面，其中一些在用液体检测程序检测时呈阳性。所使用的这2个遗传标记在检测所分析的化学诱变剂时表现出相似的敏感性（只有2个例外）。
